Closed kubu4 closed 2 years ago
Well, it appears I may have submarined a decent chunk of CEABIGR (temporarily).
Initial RNAseq alignments (BAM) for individuals was performed using untrimmed reads!
So, any analysis (including my previous ballgown analyses) should be disregarded.
ballgown
I'm in the process of re-running things. Will report back here when things are ready to test everyone's code reproducibility!
So sorry to everyone involved in this!
No problem.. most of the work is still in the phase of developing the pipelines, so it can continue, and simple insert new files. Noting there is a contingent that beliefs trimming in RNAseq is not necessary.
see paper https://academic.oup.com/nargab/article/2/3/lqaa068/5901066
Well, it appears I may have submarined a decent chunk of CEABIGR (temporarily).
Initial RNAseq alignments (BAM) for individuals was performed using untrimmed reads!
So, any analysis (including my previous
ballgown
analyses) should be disregarded.I'm in the process of re-running things. Will report back here when things are ready to test everyone's code reproducibility!
So sorry to everyone involved in this!