sr320 / ceabigr

Workshop on genomic data integration with a emphasis on epigenetic data (FHL 2022)
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Generate CIRCOS plot #70

Closed sr320 closed 9 months ago

sr320 commented 2 years ago

of gene expression, methylation, etc - maybe 1 chromosome...

kubu4 commented 2 years ago

Just a status update.

Finally getting back into the swing of things with this stuff. Here's a quick and "easy" plot of a single chromosome showing mean gene FPKM between control females (black inner ring) and exposed females (green inner ring).

Will get some methylation stuff going, as well as transcript counts or something. It's very easy to plot all of this across all chromosomes, but for testing/starting out working on just a single chromosome helps make the process a tad easier.

image

kubu4 commented 2 years ago

Here's an updated plot of single chormosome (colors describe interior rings):

I'll work on getting transcript counts plotted as well.

circos

kubu4 commented 2 years ago

@yaaminiv Here's an overly complicated plot all of the data I've managed to get into Circos histogram format. Not sure if you just want to throw it into your talk as a pretty picture/example of how we're starting to visualize data?

Description: Comparison of female C.virginica gonad data, per gene on chromosome NC_035781.1. Control samples are always the lighter, outer plots and OA-exposed are the darker, inner plots.

PURPLE: Mean gene expression (FPKM)

RED: Mean percent methylated.

BLUE: Number of transcripts.

Link to files (PNG and SVG formats): https://github.com/sr320/ceabigr/tree/main/output/figures/circos

Note: If you want to use these, please download them. These should be considered "temporary".

Circos plot showing female data: purple tracks (control/exposed fpkm), red tracks (mean methylation), blue tracks (transcript counts)