blastn error message for quiz

[yaaminiv]

I was able to create my database of differentially expressed seastar genes and now I have my .fastq datafile to compare it to. I set up the code for a blastn and I'm trying to run it, but I keep getting this error message.

I'm not sure how to fix this, or what it's referring to! My notebook can be found here.

[sr320]

You are not blasting a fasta file. You need to be. It appears you are blasting a fastq file. Blast the transcriptome- not raw reads.

On Thu, Oct 27, 2016 at 4:00 PM Yaamini Venkataraman < notifications@github.com> wrote:

I was able to create my database for blastn and I'm trying to run it, but I keep getting this error message.

[image: screen shot 2016-10-27 at 3 56 22 pm] https://cloud.githubusercontent.com/assets/22335838/19788592/21d6a8f4-9c5e-11e6-981b-2f7123855801.png

I'm not sure how to fix this, or what it's referring to! My notebook can be found here https://github.com/yaaminiv/yaaminiv-fish546-2016/blob/master/tutorials/2016-10-26-DESeq2-Tutorial-Part-2.ipynb .

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[yaaminiv]

I'm now trying to convert my .fastq file to a .fasta file in Galaxy but it looks like I don't have enough space to do so.

Should I just abandon this effort and get a O. lurida .fasta file from somewhere else?

[sr320]

please see https://github.com/yaaminiv/yaaminiv-fish546-2016/issues/1

😸

[yaaminiv]

Thanks! I didn't even get notified that the issue was created, so I didn't see it.

[sr320]

Be sure to check https://github.com/settings/notifications