Just tried to run the pipeline today, but it seems like NCBI has stopped hosting mito.nt.gz on their BLAST FTP (ftp://ftp.ncbi.nlm.nih.gov/blast/db/FASTA/mito.nt.gz) causing AAFTF to error out during the vecscreen stage. I changed my local installation to point to the RefSeq mitochondrial genome FTP instead (ftp://ftp.ncbi.nlm.nih.gov/refseq/release/mitochondrion/mitochondrion.1.1.genomic.fna.gz) and works fine, so thought I would make a pull request.
Hey,
Just tried to run the pipeline today, but it seems like NCBI has stopped hosting mito.nt.gz on their BLAST FTP (ftp://ftp.ncbi.nlm.nih.gov/blast/db/FASTA/mito.nt.gz) causing AAFTF to error out during the vecscreen stage. I changed my local installation to point to the RefSeq mitochondrial genome FTP instead (ftp://ftp.ncbi.nlm.nih.gov/refseq/release/mitochondrion/mitochondrion.1.1.genomic.fna.gz) and works fine, so thought I would make a pull request.
Cam