yimmieg
commented
5 years ago These are all multiplexed. Run GSM2560245_A contains 4 samples, run GSM2560246_B contains 4 samples and run GSM2560247_C contains 8. Run GSM2560248_2.1 andGSM2560249_2.2 contain data from 16 samples.
On Oct 10, 2018, at 12:55 AM, 2236529177 notifications@github.com wrote:
I found the paper "Multiplexed droplet single-cell rNA-sequencing using natural genetic variation" mentioned eight samples , but there are only five fastqs in EBI of SRP102802. and there are only five fastq.gz, no annotated left and right(in the website https://www.ebi.ac.uk/ena/data/view/PRJNA381100 https://www.ebi.ac.uk/ena/data/view/PRJNA381100)
and in the geo website, no genes.tsv found
https://user-images.githubusercontent.com/25199946/46720953-78e86880-cca4-11e8-97fb-78cbbd4f126a.png Thanks a lot. and do you do extral quality control of fastq files, for example, in bulk rna-seq, used softwares like seqprep, scikle.
— You are receiving this because you are subscribed to this thread. Reply to this email directly, view it on GitHub https://github.com/statgen/demuxlet/issues/30, or mute the thread https://github.com/notifications/unsubscribe-auth/AEUMf5FJW4_nCx3Y_s3Le3MDOBVd9VlCks5ujagOgaJpZM4XUxPf.
I found the paper "Multiplexed droplet single-cell rNA-sequencing using natural genetic variation" mentioned eight samples , but there are only five fastqs in EBI of SRP102802. and there are only five fastq.gz, no annotated left and right(in the website https://www.ebi.ac.uk/ena/data/view/PRJNA381100)
and in the geo website, no genes.tsv found
Thanks a lot. and do you do extral quality control of fastq files, for example, in bulk rna-seq, used softwares like seqprep, scikle.