hyunminkang
commented
5 years ago I think reads without UMIs will be ignored. There could be a workaround (e.g. modifying BAMs, allowing a --no-UMI parameter), but the software update may not happen very soon given our heavy workload.
Hyun.
Hyun Min Kang, Ph.D. Associate Professor of Biostatistics University of Michigan, Ann Arbor Email : hmkang@umich.edu
On Mon, Jun 3, 2019 at 9:54 AM PernilleYR notifications@github.com wrote:
Hi authors!
I have some single-cell ATAC seq data from 10X on which I would like to use demuxlet, however there is no UMI barcode in ATAC seq results. Is there a way to specify that there is no UMI? or should it work anyway?
The solution we thought about is to give the sequence as the UMI barcodes, then every reads except duplicates would be considered. However might be heavy as the sequences are very long.
Thank you very much!
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Hi authors!
I have some single-cell ATAC seq data from 10X on which I would like to use demuxlet, however there is no UMI barcode in ATAC seq results. Is there a way to specify that there is no UMI? or should it work anyway?
The solution we thought about is to give the sequence as the UMI barcodes, then every reads except duplicates would be considered. However might be heavy as the sequences are very long.
Thank you very much!