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statgen / demuxlet

Genetic multiplexing of barcoded single cell RNA-seq
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Understanding the naming of Doublets and Ambiguous barcodes #70

Open prashanthsama1 opened 3 years ago

prashanthsama1 commented 3 years ago

Hi,

First of all thanks for the amazing tool.

I have a very naive question regarding the naming of assigned doublets and ambiguous droplets. In one of my project after demultiplexing I see the following results. How exactly were doublets and ambiguous barcodes/cells were named, is there a significance on which sample ID appears first. For example, we can see 3 diff ambiguous droplets and 2 diff doublets, what is the difference between all these ambiguous and doublet cells.

  17  AMB-S4803-S4832-S4803/S4832
 14   AMB-S4832-S4803-S4803/S4832
  1     AMB-S4832-S4803-S4832/S4803
1046 DBL-S4803-S4832-0.500
  15   DBL-S4832-S4803-0.500
816  SNG-S4803
2109 SNG-S4832

Best , Prashanth Sama