Get reliable singlets without a proper depth seq of one donor

statgen / demuxlet

Genetic multiplexing of barcoded single cell RNA-seq

Apache License 2.0

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Get reliable singlets without a proper depth seq of one donor #74

Open vianCastelan opened 4 years ago

vianCastelan commented 4 years ago

I performed the demultiplexing of a 5 donor library, but the depth sequencing of one of my donors (call it X) is not good enough. So I'd like to know how reliable are the Singlets I'm obtaining for the rest of the donors? Is X donor adding noise to my deconvolution? How can I fix it?