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statgen / demuxlet

Genetic multiplexing of barcoded single cell RNA-seq
Apache License 2.0
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Is there any example using demuxlet with scRNA-seq platforms other than 10x? #94

Open kohjy2000 opened 2 years ago

kohjy2000 commented 2 years ago

I need to use a demuxlet to demultiplex the data generated by BD Rhapsody platform. I checked the --tag-umi and --tag-group formats of the data generated by Bhapsody, but I don't know how to enter the input in the options.

Can you give me an example?

Thank you for your cooperation.

leqi0001 commented 3 days ago

This is very late but --tag-umi MA should work for Rhapsody. However, I do notice that the run is extremely slow with this tag present. We're talking about >10-fold slower. I'm wondering if there's a reason for this. It doesn't seem to affect 10x files as much.