demuxlet output first row is not contain the barcode information

statgen / demuxlet

Genetic multiplexing of barcoded single cell RNA-seq

Apache License 2.0

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Open cacaonib opened 1 year ago

cacaonib commented 1 year ago

Hi Thank you for making good programs

I have one question using demuxlet

When I finished demuxlet, I got the three outputs (.best, single, single2)

I open the .best file using R

demuxlet <- fread("./demuxlet.best");

the first row does not contain the barcode

so, I'm curious why the first column contains just dot?

Is there any possibility that one row will get pushed out?

how to solve that problem ?

thank you

cacaonib commented 1 year ago

Using ATAC data, Also first column doesn't contain the barcode.

when I saw the output data, 1st row has the highest RD.TOTL value

Does this mean that the 1st column is the sum of PB17 reads?