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statgen / popscle

A suite of population scale analysis tools for single-cell genomics data including implementation of Demuxlet / Freemuxlet methods and auxilary tools
https://github.com/statgen/popscle/wiki
Apache License 2.0
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tutorial dataset #56

Open wxicu opened 2 years ago

wxicu commented 2 years ago

Hallo, i was running popscle demuxlet on the tutorial dataset, which detected 6 doublets. However, based on the .best file in the goole drive, the dataset has roughly 40 doublets. Why was a difference between 2 result? Does dsc-pileup change the result eventually?

Thanks and best wishes Xichen

hyunminkang commented 2 years ago

Most likely, it is because the default parameter was changed. The default parameters are not identical between versions

On Fri, Sep 9, 2022, 7:43 PM Xichen Wu @.***> wrote:

Hallo, i was running popscle demuxlet on the tutorial dataset, which detected 6 doublets. However, based on the .best file in the goole drive, the dataset has roughly 40 doublets. Why was a difference between 2 result? Does dsc-pipeup change the result eventuallu?

Thanks and best wishes Xichen

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wxicu commented 2 years ago

Thank you so much for the quick response! This would be helpful! I was struggling to have the same result.

wxicu commented 2 years ago

Sorry but another question, can I ask which parameter has changed? I am thinking maybe the alpha value in this case.