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strawlab / Miriam

Miriam is an open-source isothermal reaction instrument featuring realtime fluorescence readout in a 96-well plate format
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improve dynamic range of fluoresence sensing #1

Open astraw opened 4 years ago

astraw commented 4 years ago

Here's the result of an experiment we just ran. As can be seen, some the fluorescence values are saturated. This is likely from exceeding the dynamic range of the sensor as we are using it. It would be good if they didn't saturate.

Image Pasted at 2020-10-23 12-25

IMG_4348

astraw commented 3 years ago

Perhaps reducing the gain by a factor of 2 would be sufficient.

astraw commented 3 years ago

It might be possible to obtain a non-saturated reading of highly fluorescent wells by pulsing the LEDs very briefly. (Either as a single pulse or a low duty cycle on a PWM LED illumination program which the ADC and photodiode integrate over.)