What's the purpose of this construct?
Integrase expression construct. Primary goal is to establish an efficient protocol for sequential insertion of DNA fragments into a backbone for (1) bar-coding purposes and (2) combinatorial pathway assembly.
Discussion & Comments
Judging from literature BxB1 is, so far, the best-performing Serine recombinase for cloning purposes. It features fast kinetics and near 100% recombination efficiency without any excision activity.
What's the purpose of this construct? Integrase expression construct. Primary goal is to establish an efficient protocol for sequential insertion of DNA fragments into a backbone for (1) bar-coding purposes and (2) combinatorial pathway assembly.
Discussion & Comments Judging from literature BxB1 is, so far, the best-performing Serine recombinase for cloning purposes. It features fast kinetics and near 100% recombination efficiency without any excision activity.
Implementation Progress