installation error

[cnxiaobo94]

Hi, I met a problem with installation of msmc2. Before this installation, DMD and gsl have been installed correctly, and the path of gsl also has been added to makefile of msmc2. Could you do me a favor?

(base) xiaobo@xiaoboLinux:~/bioinformatics/msmc2-master$ make dmd -O /home/xiaobo/bioinformatics/gsl-2.6/lib/libgsl.a /home/xiaobo/bioinformatics/gsl-2.6/lib/libgslcblas.a -odbuild/test -ofbuild/release/msmc2 model/data.d model/gsl_matrix_vector.d model/propagation_core.d model/psmc_hmm.d model/psmc_model.d model/stateVecAllocator.d model/stateVec.d model/time_intervals.d model/transition_rate.d powell.d brent.d maximization_step.d expectation_step.d msmc2.d logger.d /home/xiaobo/bioinformatics/miniconda3/bin/../lib/gcc/x86_64-conda-linux-gnu/11.2.0/../../../../x86_64-conda-linux-gnu/bin/ld: /home/xiaobo/bioinformatics/miniconda3/bin/../lib/gcc/x86_64-conda-linux-gnu/11.2.0/../../../../x86_64-conda-linux-gnu/lib/../lib64/libgcc_s.so.1: undefined reference to `memcpy@GLIBC_2.14' collect2: error: ld returned 1 exit status Error: linker exited with status 1 make: *** [Makefile:20：build/release/msmc2] 错误 1

[stschiff]

It seems the linker can't fine GLIBC. I can't really support that. Have you tried one of the precompiled executables in releases? https://github.com/stschiff/msmc2/releases/tag/v2.1.4

[tcztzy]

1. I noticed that your miniconda3 used ld and libgcc_s.so.1 from base environment, this way is not recommended. I created a new environment and compiled msmc2 successful. Notice that I replace dmd which ldc2 because the dmd in conda-forge channel doesn't the requirement to build source codes. here is my environment.yml

   name: msmc2
   channels:
   - conda-forge
   - bioconda
   - defaults
   dependencies:
   - gsl=2.7=he838d99_0
   - ldc=1.28.1=hcf88599_0
   - msmc2=2.1.4=hdfd78af_0
   - binutils=2.40=hdd6e379_0

   You can reproduce the environment via conda env create -f environment.yml. After creating environment, assume the environment path is ~/miniconda3/envs/msmc2, you should modify the Makefile via

   $ sed -i 's/dmd/ldc2/g' Makefile
   $ sed -i 's/\/usr\/local\/lib/~\/miniconda3\/envs\/msmc2\/lib/g' Makefile

   Or even tricky, create symbol links

   $ ln -s $(which ldc2) $(dirname -- $(which ldc2))/dmd
   $ ln -s ~/miniconda3/envs/msmc2/lib/libgsl.a /usr/local/lib
   $ ln -s ~/miniconda3/envs/msmc2/lib/libgslcblas.a /usr/local/lib

2. LIFESAVER: You can download the precompiled executables from release page. There is also msmc2 in bioconda channel.

REFERENCES:

• https://docs.conda.io/projects/conda/en/latest/user-guide/tasks/manage-environments.html
• https://docs.conda.io/projects/conda-build/en/latest/resources/compiler-tools.html

[cnxiaobo94]

1. I noticed that your miniconda3 used ld and libgcc_s.so.1 from base environment, this way is not recommended. I created a new environment and compiled msmc2 successful. Notice that I replace dmd which ldc2 because the dmd in conda-forge channel doesn't the requirement to build source codes. here is my environment.yml

name: msmc2
channels:
  - conda-forge
  - bioconda
  - defaults
dependencies:
  - gsl=2.7=he838d99_0
  - ldc=1.28.1=hcf88599_0
  - msmc2=2.1.4=hdfd78af_0
  - binutils=2.40=hdd6e379_0

You can reproduce the environment via conda env create -f environment.yml. After creating environment, assume the environment path is ~/miniconda3/envs/msmc2, you should modify the Makefile via

$ sed -i 's/dmd/ldc2/g' Makefile
$ sed -i 's/\/usr\/local\/lib/~\/miniconda3\/envs\/msmc2\/lib/g' Makefile

Or even tricky, create symbol links

$ ln -s $(which ldc2) $(dirname -- $(which ldc2))/dmd
$ ln -s ~/miniconda3/envs/msmc2/lib/libgsl.a /usr/local/lib
$ ln -s ~/miniconda3/envs/msmc2/lib/libgslcblas.a /usr/local/lib

2. LIFESAVER: You can download the precompiled executables from release page. There is also msmc2 in bioconda channel.

REFERENCES:

• https://docs.conda.io/projects/conda/en/latest/user-guide/tasks/manage-environments.html
• https://docs.conda.io/projects/conda-build/en/latest/resources/compiler-tools.html

Thank you for your very careful explanation. I will do it according to your process.

[charlesfeigin]

I'm having a somewhat similar issue:

dmd -O /usr/local/lib/libgsl.a /usr/local/lib/libgslcblas.a -odbuild/test -ofbuild/release/msmc2 model/data.d model/gsl_matrix_vector.d model/propagation_core.d model/psmc_hmm.d model/psmc_model.d model/stateVecAllocator.d model/stateVec.d model/time_intervals.d model/transition_rate.d powell.d brent.d maximization_step.d expectation_step.d msmc2.d logger.d /usr/bin/ld: cannot find /usr/local/lib/libgsl.a: No such file or directory /usr/bin/ld: cannot find /usr/local/lib/libgslcblas.a: No such file or directory collect2: error: ld returned 1 exit status Error: linker exited with status 1 make: *** [Makefile:20: build/release/msmc2] Error 1

I'm happy to use the precompiled binary to run msmc2, but this doesn't appear to come with the necessary scripts like bamcaller.py. The source code also doesn't seem to have the scripts when I git clone or download the .tar.gz. How is the best way to use the precompiled binary and also get the scripts needed to prepare inputs?

[tcztzy]

I'm having a somewhat similar issue:

dmd -O /usr/local/lib/libgsl.a /usr/local/lib/libgslcblas.a -odbuild/test -ofbuild/release/msmc2 model/data.d model/gsl_matrix_vector.d model/propagation_core.d model/psmc_hmm.d model/psmc_model.d model/stateVecAllocator.d model/stateVec.d model/time_intervals.d model/transition_rate.d powell.d brent.d maximization_step.d expectation_step.d msmc2.d logger.d /usr/bin/ld: cannot find /usr/local/lib/libgsl.a: No such file or directory /usr/bin/ld: cannot find /usr/local/lib/libgslcblas.a: No such file or directory collect2: error: ld returned 1 exit status Error: linker exited with status 1 make: *** [Makefile:20: build/release/msmc2] Error 1

You might check the Makefile

https://github.com/stschiff/msmc2/blob/5fc12bcd3afc74569cfc9d457b74497fbf441012/Makefile#L1

I'm happy to use the precompiled binary to run msmc2, but this doesn't appear to come with the necessary scripts like bamcaller.py. The source code also doesn't seem to have the scripts when I git clone or download the .tar.gz. How is the best way to use the precompiled binary and also get the scripts needed to prepare inputs?

You might check the README

This program implements MSMC2, a method to infer population size history and population separation history from whole genome sequencing data. For a general guide, see here.

[stschiff]

For the record, a user contributed an MSMC2 package to conda: https://bioconda.github.io/recipes/msmc2/README.html?highlight=msmc2#package-package%20'msmc2'