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stuart-lab / signac

R toolkit for the analysis of single-cell chromatin data
https://stuartlab.org/signac/
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FindTransferAnchors error !! #1846

Open synatkeamsk opened 1 day ago

synatkeamsk commented 1 day ago

Dear Developers,

I have run script from Signac tutorial and got error when I reach the below part 

pbmc_rna <- readRDS("pbmc_10k_v3.rds")
pbmc_rna <- UpdateSeuratObject(pbmc_rna)
transfer.anchors <- FindTransferAnchors(
  reference = pbmc_rna,
  query = pbmc,
  reduction = 'cca'
)

I got the following error !! 

Error: No features to use in finding transfer anchors. To troubleshoot, try explicitly providing features to the features parameter and ensure that they are present in both reference and query assays.

I tried to troubleshooted it by looking whether people have encountered the same issue, but I could not fix it. I am wondering whether you could help look and suggest some solutions.

Kind Regards,

synat