Closed FraMont1 closed 2 years ago
I wasn't able to reproduce this issue. Can you try running this code and see if you have any errors:
library(GenomeInfoDb)
whole.genome <- as(object = seqinfo(x = EnsDb.Hsapiens.v75), Class = "GRanges")
whole.genome <- keepStandardChromosomes(whole.genome, pruning.mode = "coarse")
annot <- biovizBase::crunch(obj = EnsDb.Hsapiens.v75, which = whole.genome[1],
columns = c("tx_id", "gene_name", "gene_id",
"gene_biotype"))
If so, then the issue is likely with some Bioconductor dependencies rather than Signac itself.
Thank you for your answer.
I ran the code and I obtained the same error
Fetching data...OK Parsing exons...OK Defining introns...OK Defining UTRs...Error in h(simpleError(msg, call)) : error in evaluating the argument 'x' in selecting a method for function 'splitAsList': error in evaluating the argument 'x' in selecting a method for function 'end': error in evaluating the argument 'args' in selecting a method for function 'do.call':
is this a possible issue due to Bioconductor dependencies?
Yes, you can get support for this on the bioconductor site: https://support.bioconductor.org/
Hi,
Thank you for creating such a useful tool.
I am trying to reproduce the tutorial: Analyzing PBMC scATAC-seq using the following code (the same of the tutorial)
but I obtain this error
I have tried to see if someone else had this issue, I have tried to install different version of Seurat and Signac but I did not find how to figure out.
Any help would be greatly appreciated. Best wishes Francesco
This is my session info: