why-in-Shanghaitech
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5 years ago This is for issue comment testing.
Open why-in-Shanghaitech opened 5 years ago
why-in-Shanghaitech
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5 years ago This is for issue comment testing.
why-in-Shanghaitech
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5 years ago Local test comment.
why-in-Shanghaitech
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5 years ago Local test comment for username and password. And Button.
why-in-Shanghaitech
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5 years ago 1 0:00:00,330 --> 0:00:02,580 oh 哦
2 0:00:05,370 --> 0:00:10,679 the overall goal of this procedure is to separate DNA fragments of varying sizes 这个程序的总体目标是分离不同大小的DNA片段。
3 0:00:11,550 --> 0:00:16,859 this is accomplished by first preparing a shell with the appropriate concentration of gross and a flora 这是通过首先准备一个具有适当浓度的毛和植物群的壳来完成的。
4 0:00:16,864 --> 0:00:17,969 some die 有些人死了
5 0:00:18,420 --> 0:00:23,730 the second step is to pour the gel into an appropriate mold and allow it to set 第二步是将凝胶倒入适当的模具并使其凝固。
6 0:00:24,088 --> 0:00:28,710 next the samples are loaded into the gel and a current is applied 接着，将样品加载到凝胶中，并施加电流。
7 0:00:29,280 --> 0:00:34,590 the final step is to visualize the separated DNA fragments under ultraviolet light 最后一步是在紫外光下可视化分离的DNA片段。
8 0:00:35,609 --> 0:00:40,920 summary gross for races is used in all situations where the route 在路线的所有情况下，都使用比赛总成绩汇总表。
9 0:00:40,923 --> 0:00:44,070 separation of DNA fragments is required 需要分离DNA片段
10 0:00:45,658 --> 0:00:50,490 the main advantage of this technique over existing method of the time 这项技术相对于现有的时间方法的主要优势
11 0:00:51,689 --> 0:00:54,240 density gradient sent to vacation 密度梯度发送到假期
12 0:00:55,020 --> 0:00:55,890 provides 提供
13 0:00:56,368 --> 0:00:58,380 realising a Benz 实现奔驰
14 0:00:58,439 --> 0:01:03,750 it also enables us to determine the exact size of exact size of the DNA 它还使我们能够确定DNA的准确大小。
15 0:01:04,078 --> 0:01:06,959 when seperated concurrently without DNA marker 同时分离时没有DNA标记
16 0:01:07,438 --> 0:01:10,260 method is essential in life science research 方法在生命科学研究中是必不可少的
17 0:01:10,349 --> 0:01:15,420 such as in the cloning of genes and the purification and sequencing of DNA molecules 例如基因的克隆和DNA分子的纯化和测序
18 0:01:15,780 --> 0:01:21,090 though this method is generally used to separate DNA fragments between one hundred base pairs 尽管这种方法通常用于分离100个碱基对之间的DNA片段
19 0:01:21,840 --> 0:01:23,400 five kilo basis 5公斤
20 0:01:23,549 --> 0:01:28,349 you can also be modified to separate DNA fragments up to ten mega bases in size 你也可以被修改来分离DNA片段，大小可达十个巨型碱基。
21 0:01:33,688 --> 0:01:38,579 girls gels are prepared using a weight over volume percentage solution 女孩凝胶是用重量比体积百分比溶液配制的。
22 0:01:38,700 --> 0:01:44,009 the concentration of the gel will depend on the sizes of the DNA fragments to be separate 凝胶的浓度将取决于分离的DNA片段的大小。
23 0:01:44,878 --> 0:01:47,158 begin the procedure for making a joke 开始开玩笑的程序
24 0:01:47,310 --> 0:01:51,390 weigh out the appropriate mass of arrows into an irl in Myer flask 将适当质量的箭头称入Myer烧瓶中的IRL中。
25 0:01:52,049 --> 0:01:55,289 the appropriate volume of running buffer to the flask 向烧瓶输送缓冲液的适当体积
26 0:01:55,378 --> 0:02:00,450 the volume of the buffer should not be greater than one third of the capacity of the flask 缓冲液的体积不应大于烧瓶容量的三分之一。
27 0:02:00,629 --> 0:02:01,950 swirled to mix 旋转混合
28 0:02:02,760 --> 0:02:07,650 melt the gross buffer mixture by heating in a microwave at maximum power 在微波炉中以最大功率加热，熔化总的缓冲混合物。
29 0:02:08,128 --> 0:02:13,080 thirty second intervals remove the flask and swirl the contents to mix well 每隔30秒取出烧瓶，旋转内容物使其充分混合。
30 0:02:13,378 --> 0:02:16,408 repeat until the arrows has completely dissolved 重复直到箭头完全溶解
31 0:02:17,038 --> 0:02:17,670 next 下一个
32 0:02:17,788 --> 0:02:23,038 bromide to a concentration of zero point five micrograms per milliliter 溴化物浓度为0.5微克/毫升
33 0:02:23,280 --> 0:02:28,590 it is important to note that if video bromide is a carcinogen so gloves should always be worn 重要的是要注意，如果溴化视频是致癌物，则应始终戴手套。
34 0:02:28,593 --> 0:02:31,530 when handling gels containing a video bromide 处理含有视频溴化物的凝胶时
35 0:02:32,128 --> 0:02:37,110 allow the gross to cool by incubation in a sixty five degrees Celsius water bath 让毛重在65摄氏度的水浴中孵化冷却。
36 0:02:37,438 --> 0:02:40,348 failure to do so will warp the gel tray 如果不这样做，就会翘曲凝胶托盘。
37 0:02:41,038 --> 0:02:46,348 while the agro says cooling prepare the gel mold by placing the gel tray into the casting app or 而农产品说，冷却通过将凝胶托盘放入铸造应用程序来制备凝胶模具。
38 0:02:47,280 --> 0:02:51,658 alternatively one may take the open edges of jail try to create a mould 另一种方法是利用监狱的开放边缘来制造模具。
39 0:02:52,139 --> 0:02:55,829 appropriate comb into the gel mold to create the Wells 适当梳入凝胶模具创造威尔斯
40 0:02:56,670 --> 0:02:59,400 the molded into the gel mold 模压成胶模
41 0:02:59,579 --> 0:03:02,669 allow the Agra to set at room temperature 允许AGRA在室温下设置
42 0:03:03,210 --> 0:03:06,509 once the agro says set remove the comb 一旦农说设置，删除梳
43 0:03:06,718 --> 0:03:09,628 if the gel is not going to be used immediately 如果凝胶不能立即使用
44 0:03:09,718 --> 0:03:14,098 rapid plastic wrap and store at four degrees Celsius until use 快速塑料包装，并在4摄氏度下存放直至使用
45 0:03:14,669 --> 0:03:19,288 if the job is going to be used immediately place the gel in the gel box 如果工作要立即使用，将凝胶放入凝胶盒中。
46 0:03:24,598 --> 0:03:26,188 to begin this procedure 开始这个程序
47 0:03:26,193 --> 0:03:30,090 gel loading dye to the DNA samples to be separated 待分离的DNA样品的凝胶负载染料
48 0:03:30,598 --> 0:03:34,650 loading diet is typically made at a six x concentration 负荷饮食通常是在六倍浓度下进行的。
49 0:03:35,218 --> 0:03:38,610 program the power supplied to the desired voltage 将电源编程到所需电压
50 0:03:39,210 --> 0:03:44,158 now add enough fronting buffer into the gel box to cover the surface of the gel 现在在凝胶盒中加入足够的前置缓冲液来覆盖凝胶表面。
51 0:03:44,340 --> 0:03:49,348 it is important to use the same running buffer as the one used to prepare the jail 使用与监狱准备相同的缓冲区是很重要的。
52 0:03:49,920 --> 0:03:54,930 attached the leads of the gel box to the power supply and turn on the power supply 将胶盒的引线连接到电源并接通电源。
53 0:03:55,378 --> 0:03:59,218 verify both the gel box and the power supply are working 验证凝胶盒和电源是否工作
54 0:03:59,520 --> 0:04:04,020 new appearance of bubbles at the electrodes indicates that current is passing through 电极上新出现的气泡表明电流正在通过
55 0:04:04,348 --> 0:04:09,658 since our has tend to shake a little natural it may be difficult to get a 因为我们的振动有点自然，所以很难得到
56 0:04:09,663 --> 0:04:10,590 pipe 管
57 0:04:11,310 --> 0:04:12,060 oh well 哦，好吧
58 0:04:12,688 --> 0:04:14,908 one way to prevent your hand from shaking 一种防止手颤抖的方法
59 0:04:16,470 --> 0:04:17,910 on the other arm 另一只手臂
60 0:04:18,480 --> 0:04:19,110 a job 一份工作
61 0:04:20,189 --> 0:04:22,110 the lid of the box 盒子的盖子
62 0:04:22,439 --> 0:04:26,310 slowly and carefully load the DNA samples into the gel 慢慢仔细地把DNA样本装入凝胶中。
63 0:04:26,939 --> 0:04:32,250 loading dye in the sample allows the sample to sink into the jail and will help to track how 在样本中加入染料可以让样本沉入监狱，并有助于跟踪
64 0:04:32,254 --> 0:04:33,750 are the sample has travelled 样品是否已移动？
65 0:04:34,439 --> 0:04:39,389 DNA size marker should always be loaded along with the experimental samples DNA大小标记应该和实验样本一起装载。
66 0:04:40,139 --> 0:04:41,490 replace the lid 更换盖子
67 0:04:41,908 --> 0:04:46,860 double check that the electrodes are plugged into the correct slots in the power supply 再次检查电极是否插入电源的正确插槽中。
68 0:04:47,430 --> 0:04:49,230 turn on the power 打开电源
69 0:04:49,590 --> 0:04:53,519 run the jail until the dye has migrated to an appropriate distance 坐牢直到染料移到适当的距离
70 0:04:58,829 --> 0:05:04,139 when electro for recess has completed turn off the power supply and remove the lid of the job 当凹处的电气设备完成后，关闭电源并卸下工作盖。
71 0:05:05,399 --> 0:05:10,709 remove the gel from the gel box and drain off excess buffer on the surface of the gel 从凝胶盒中取出凝胶，并在凝胶表面上排出多余的缓冲液。
72 0:05:11,459 --> 0:05:16,079 place the gel tray on paper towels to absorb any remaining running buffer 将凝胶托盘放置在纸巾上，以吸收任何剩余的运行缓冲液。
73 0:05:16,680 --> 0:05:18,899 visualize the DNA fragments 想象DNA片段
74 0:05:19,079 --> 0:05:23,850 remove the gel from the gel tray and expose the jail to ultraviolet light 从凝胶托盘中取出凝胶，将监狱暴露于紫外线。
75 0:05:24,300 --> 0:05:28,199 this is most commonly done using a gel documentation system 这是最常用的凝胶文档系统。
76 0:05:28,829 --> 0:05:32,610 DNA bands should show up as orange fluorescent fans DNA带应该以橙色荧光扇子的形式出现。
77 0:05:32,939 --> 0:05:34,500 take a picture of the gel 拍一张凝胶的照片
78 0:05:35,040 --> 0:05:40,350 at the end of the experiment properly dispose of the gel and running buffer her institution 实验结束后，妥善处理凝胶和缓冲液。
79 0:05:40,355 --> 0:05:41,490 relations 关系
80 0:05:46,199 --> 0:05:51,509 this figure represents a typical result after gel electrophoresis 此图代表凝胶电泳后的典型结果。
81 0:05:51,629 --> 0:05:52,860 PC our products PC我们的产品
82 0:05:53,730 --> 0:05:59,040 after separation the resulting DNA fragments are visible as clearly defined bands 分离后，所得到的DNA片段以清晰的条带的形式可见。
83 0:05:59,850 --> 0:06:05,160 DNA standard or ladder should be separated to a degree that allows for the useful determination DNA标准品或梯子的分离程度应允许有用的测定。
84 0:06:05,165 --> 0:06:07,110 of the sizes of sample bands 样品带的尺寸
85 0:06:07,769 --> 0:06:09,028 in this example 在这个例子中
86 0:06:09,180 --> 0:06:12,569 DNA fragments of seven hundred and sixty five base pairs 七百六十五碱基对的DNA片段
87 0:06:12,778 --> 0:06:18,088 eight hundred and eighty base pairs in one thousand twenty-two base pairs are separated on a one 八百八十个碱基对在一千二二个碱基对中被分开在一个碱基对上。
88 0:06:18,095 --> 0:06:22,170 five percent gross gel with the two log DNA ladder 百分之五双凝胶DNA梯形凝胶
89 0:06:25,889 --> 0:06:31,199 while attempting to procedure is important to remember to double check the placement of the 当尝试进行手术时，记住要仔细检查
90 0:06:31,204 --> 0:06:31,860 natural 自然的
91 0:06:34,829 --> 0:06:35,759 sure that's the 当然，这就是
92 0:06:36,959 --> 0:06:38,310 novel right direction 新的正确方向
93 0:06:40,319 --> 0:06:44,040 should have a good understanding of how to prepare an agro self 应该很好地了解如何准备一个农业自我
94 0:06:44,730 --> 0:06:46,920 DNA fragments bearing sizes 带有大小的DNA片段
95 0:06:47,610 --> 0:06:48,209 I 我
96 0:06:48,509 --> 0:06:49,800 images of your 您的图像
97 0:06:50,879 --> 0:06:53,040 forget that working with the video bromide 忘了使用视频溴化物
98 0:06:55,470 --> 0:06:56,158 awww 空中武器战
99 0:06:57,149 --> 0:06:57,750 loves 爱
100 0:06:59,759 --> 0:07:00,899 should always be taken with her 应该一直带着她
This is for issue testing.