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stuckyb / seqtrace

User-friendly software for viewing and processing Sanger DNA sequencing trace files.
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filename formatting for auto-grouping #16

Open bwprice opened 3 years ago

bwprice commented 3 years ago

Files from our sequencing facility have filenames that extend past the "F" or "R" direction flag (e.g. A1_R_096_B23.ab1), SeqTrace only recognised pairs of tracefiles when "_F" or "_R" was the last part of the filename (e.g. A1_R.ab1). This requirement needs to be made clearer in the help text. Otherwise excellent!

stuckyb commented 3 years ago

Thanks for this comment, @bwprice. As long as everything in the forward/reverse file names matches besides the forward/reverse identifers, the program should be able to match them up. E.g., "a1_R_id23.ab1" and "a1_F_id23.ab1" should pair up correctly. If any other parts of the names do not match, seqtrace won't be able to pair the files up. E.g., "a1_R_id23.ab1" and "a1_F_id24.ab1" would not be paired. Otherwise, it doesn't matter where in the file name the "_F" and "_R" (or whatever forward/reverse identifiers you use) are located. You might double check that the file names for each pair matched exactly besides the forward/reverse flags.

bwprice commented 3 years ago

Thanks @stuckyb - the filenames don't match exactly direct from the sequencer, but I can deal with that by trimming the ends in bulk.