Open Rezwanuzzaman opened 3 months ago
Can you send me the content of the file you passed as an argument to --cytobandsFile
as well as the first fusion of the fusions file?
Which version of Arriba is this?
@suhrig I use Arriba 2.4.0. --fusions=fusions.tsv --cytobands=database/cytobands_hg19_hs37d5_GRCh37_v2.4.0.tsv
Can you send me the first fusion of the fusions file (AGAP3:BRAF)?
AGAP3 BRAF +/+ -/- 7:151128684 7:140787584 CDS/splice-site CDS/splice-site inversion 7 11 1 378 486 high in-frame . PH_domain(28%),Ras_family(100%)|Protein_kinase_domain(100%) . . ENSG00000133612 ENSG00000157764 ENST00000463381 ENST00000496384 downstream downstream duplicates(82) CGGGAGAAGAAGGCTGCCGAGTGCAAGGTGGACAGCATCGGGAGCGGCCGCGCCATCCCCATCAAGCAGGGGATCCTGCTAAAGCGGAGCGGCAAGTCCCTGAACAAGGAGTGGAAGAAGAAGTATGTGACGCTCTGTGACAACGGGCTGCTCACCTATCACCCCAGCCTGCAT|GACTTGATTAGAGACCAAGGATTTCGTGGTGATGGAGGATCAACCACAGGTTTGTCTGCTACCCCCCCTGCCTCATTACCTGGCTCACTAACTAACGTGAAAGCCTTACAGAAATCTCCAGGACCTCAGCGAGAAAGGAAGTCATCTTCATCCTCAGAAGACAGGAATCGAATG___AAAACACTTGGTAGACGGGACTCGAGTGATGATTGGGA REKKAAECKVDSIGSGRAIPIKQGILLKRSGKSLNKEWKKKYVTLCDNGLLTYHPSLH|DLIRDQGFRGDGGSTTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSEDRNRMKTLGRRDSSDDW K00102:513:HHM7KBBXY:7:1109:15158:33949,K00102:513:HHM7KBBXY:7:1115:18588:40174,K00102:517:HJTWNBBXY:1:1101:5690:7416,K00102:517:HJTWNBBXY:1:1110:29203:1490,K00102:517:HJTWNBBXY:1:1118:2909:4040,K00102:517:HJTWNBBXY:1:1122:16204:2246,K00102:517:HJTWNBBXY:1:1122:2605:31962,K00102:517:HJTWNBBXY:1:1208:23551:47999,K00102:517:HJTWNBBXY:1:1223:6066:44394,K00102:517:HJTWNBBXY:1:2114:11292:22186,K00102:517:HJTWNBBXY:2:2107:8714:30978,K00102:517:HJTWNBBXY:2:2122:15818:6361,K00102:517:HJTWNBBXY:3:1124:27702:12708,K00102:517:HJTWNBBXY:3:1227:11231:7169,K00102:517:HJTWNBBXY:3:2216:14468:44640,K00102:517:HJTWNBBXY:4:1105:12225:13253,K00102:517:HJTWNBBXY:4:1125:2067:36939,K00102:517:HJTWNBBXY:4:1126:11221:38803,K00102:517:HJTWNBBXY:4:1126:12418:2439,K00102:517:HJTWNBBXY:4:1204:5132:10598,K00102:517:HJTWNBBXY:4:1212:2199:8752,K00102:517:HJTWNBBXY:4:1212:29802:41809,K00102:517:HJTWNBBXY:4:2124:22374:48597,K00102:517:HJTWNBBXY:5:2201:27499:37114,K00102:517:HJTWNBBXY:5:2213:14905:7486,K00102:517:HJTWNBBXY:5:2218:31649:13042,K00102:517:HJTWNBBXY:5:2220:7517:38222,K00102:517:HJTWNBBXY:6:1104:9759:7750,K00102:517:HJTWNBBXY:6:1111:25033:10897,K00102:517:HJTWNBBXY:6:2203:24667:7451,K00102:517:HJTWNBBXY:6:2215:31274:16436,K00102:517:HJTWNBBXY:7:2202:16173:14713,K00102:523:HK7FLBBXY:1:2116:14478:13886,K00102:523:HK7FLBBXY:1:2116:15229:13113,K00102:523:HK7FLBBXY:1:2224:10013:47014,K00102:523:HK7FLBBXY:1:2228:10125:16084,K00102:523:HK7FLBBXY:2:1113:17614:13939,K00102:523:HK7FLBBXY:2:2207:30655:10510,K00102:523:HK7FLBBXY:2:2225:12936:29641,K00102:523:HK7FLBBXY:3:1227:22414:3793,K00102:523:HK7FLBBXY:4:1207:26423:10669,K00102:523:HK7FLBBXY:4:1228:21887:40965,K00102:523:HK7FLBBXY:4:2115:10906:8928,K00102:523:HK7FLBBXY:4:2214:9851:45010,K00102:523:HK7FLBBXY:4:2227:16001:10862,K00371:401:HFH3NBBXY:3:2104:26666:22379,K00371:401:HFH3NBBXY:4:1214:10439:38046,K00371:401:HFH3NBBXY:4:1214:16244:11987,K00371:401:HFH3NBBXY:4:2110:12266:44060,K00371:401:HFH3NBBXY:4:2208:9810:43708,K00371:401:HFH3NBBXY:5:1125:12794:4004,K00371:401:HFH3NBBXY:5:1212:30228:41528,K00371:401:HFH3NBBXY:5:2122:23155:21571,K00371:401:HFH3NBBXY:5:2221:25986:11671,K00371:401:HFH3NBBXY:6:1126:28909:32279,K00371:401:HFH3NBBXY:6:1201:2676:42038,K00371:401:HFH3NBBXY:6:1203:20791:13570,K00371:401:HFH3NBBXY:6:1206:4553:35620,K00371:401:HFH3NBBXY:7:2102:9222:2949,K00371:401:HFH3NBBXY:7:2110:14093:5165,K00371:401:HFH3NBBXY:8:2124:11008:23628,K00102:513:HHM7KBBXY:7:1104:23033:11126,K00102:513:HHM7KBBXY:7:1203:31050:42108,K00102:513:HHM7KBBXY:7:1214:8917:9631,K00102:513:HHM7KBBXY:7:1222:27225:13957,K00102:517:HJTWNBBXY:1:1101:31527:31154,K00102:517:HJTWNBBXY:2:1215:24190:27232,K00102:517:HJTWNBBXY:2:1223:3397:34002,K00102:517:HJTWNBBXY:2:1223:4219:32859,K00102:517:HJTWNBBXY:2:2117:5000:36218,K00102:517:HJTWNBBXY:3:2112:15737:32279,K00102:517:HJTWNBBXY:3:2210:6471:9789,K00102:517:HJTWNBBXY:4:2107:3427:45942,K00102:517:HJTWNBBXY:4:2208:31253:48438,K00102:517:HJTWNBBXY:5:1203:17452:18616,K00102:517:HJTWNBBXY:8:1127:3792:36059,K00102:517:HJTWNBBXY:8:1222:10206:9578,K00102:517:HJTWNBBXY:8:2208:22364:41053,K00102:517:HJTWNBBXY:8:2216:31010:36552,K00102:523:HK7FLBBXY:1:1215:17543:1930,K00102:523:HK7FLBBXY:1:1218:23926:39436,K00102:523:HK7FLBBXY:1:1228:31071:28991,K00102:523:HK7FLBBXY:2:1114:14864:46205,K00102:523:HK7FLBBXY:2:1114:14976:46012,K00102:523:HK7FLBBXY:2:2220:16244:42407,K00102:523:HK7FLBBXY:3:2206:2869:24085,K00371:401:HFH3NBBXY:4:2111:25378:43919,K00371:401:HFH3NBBXY:4:2121:9303:31962,K00371:401:HFH3NBBXY:5:2101:2747:7767,K00371:401:HFH3NBBXY:6:1207:3062:3952,K00371:401:HFH3NBBXY:6:2218:27123:40297,K00371:401:HFH3NBBXY:7:2119:16062:42618,K00371:401:HFH3NBBXY:8:1108:11160:17632,K00371:401:HFH3NBBXY:8:2217:11180:12779,K00102:513:HHM7KBBXY:7:2219:26880:41880,K00102:517:HJTWNBBXY:3:1113:11464:30890,K00102:517:HJTWNBBXY:3:1113:11495:30732,K00102:517:HJTWNBBXY:5:2118:20841:47313,K00102:523:HK7FLBBXY:2:2210:23104:24823,K00371:401:HFH3NBBXY:8:1214:22749:25720,K00371:401:HFH3NBBXY:8:2224:5923:12954
I cannot reproduce the error. Which GTF file did you use to run Arriba and which one did you use to run draw_fusions.R
?
Please look --
./draw_fusions.R \ --fusions=...../sample.arriba.fusions.tsv \ --alignments=..../sample.sortedByCoord.out.bam \ --output=sample.fusions.pdf \ --annotation=.../rnafusion_3.0.0/REFERENCES/ensembl/Homo_sapiens.GRCh38.102.gtf \ --cytobands=..../rnafusion_3.0.0/REFERENCES/arriba/cytobands_mm10_GRCm38_v2.4.0.tsv \ --proteinDomains=.../rnafusion_3.0.0/REFERENCES/arriba/protein_domains_hg38_GRCh38_v2.4.0.gff3
In the beginning you wrote that you used cytobands_hg19_hs37d5_GRCh37_v2.4.0.tsv
for the cytobands file. Now you write that you used cytobands_mm10_GRCm38_v2.4.0.tsv
. What did you actually do? Regardless, both are wrong. The fusions file is based on hg38/GRCh38. All the input files of draw_fusions.R
should have GRCh38
in the name. I have no problems running the script with the correct input files and your fusions file.
Do you need any further help on this?
Loading annotation Read 3010595 records Loading protein domains Read 134356 records Drawing fusion #1: AGAP3:BRAF Error in text.default(tip, y + 0.03, bandName, cex = fontSize, adj = c(0.5, : zero-length 'labels' specified Calls: drawIdeogram -> text -> text.default Execution halted