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sunjiamin0824 / CRISPR-Local

A local tool for high-throughput CRISPR single-guide RNA (sgRNA) design in plants.
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recommend to use conda env to run CRISPR-Local #3

Open chongjing opened 4 months ago

chongjing commented 4 months ago

Just to leave a comment for anyone who plan to identify gRNA in non-reference genomes.

I got same issue as this one, #2 , it seems the version of scikit-learn was the problem, can't figure out after long time debugging. Finally, I found the authors uploaded their miniconda2 env here . So I downloaded it, tar -xvzf, then activate this env source /public/home/haoliu/opt/miniconda2/bin/activate (of course some path should be changed). Finally, CRISPR-Local ran successfully using this env for non-reference genome.

biopig commented 1 month ago

我想问一下，您具体是怎么修改的呀

biopig commented 1 month ago

@chongjing

chongjing commented 3 weeks ago

我想问一下，您具体是怎么修改的呀

你将整个conda 环境下载后，运行 source /PATH/to/public/home/haoliu/opt/miniconda2/bin/activate ，就可以了，注意加上指向/public/home/haoliu/opt/miniconda2/bin/activate的路径。