Thanks for developing this useful tool! Here I met the wired issue: althought the RD-build.pl run successfully, the reference.database.txt was empty. And the only possible message I can find was the "sort: 多字符标签“$\t”" in log file (see blow)
Welcome to CRISPR-Local
---A local single-guide RNA (sgRNA) design tool for non-reference plant genomes.
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Version : 1.0
Copyright : Free software
Author : Jiamin Sun
Email : sunjm0824@webmail.hzau.edu.cn
# Today : Wed Jun 26 14:59:22 2024
# Designing mode:cas9
# Program RD-build: CRISPR sgRNA design by using reference genome.
# Start program RD-build........
# Step1: Reading fasta file.
# Genome fasta parsed.
# Step2: Reading GFF3 file.
# GFF3 file parsed.
# Step3: Extracting potential off-target sites.
# Potential off-target sites extracted.
# Step4: Extracting possible sgRNA.
# Possible sgRNA extracted.
# Step5: Creating index file.
# Index file created.
# Step6: Calculates the Rule set 2 score for the given 30-mer sgRNA.
# Step7: Mapping Possible sgRNA to potential off-target sites.
# Step8: Format seqmap result file.
# Step9: Calculates the Cutting Frequency Determination score.
# Step10: Merging and filtering the CFD result.
sort: 多字符标签“$\t”
# Total time consumption is 17045 second.
Done!
# Your job is done, open Plo.crispr_p.reference.database.txt to check result
Thanks for developing this useful tool! Here I met the wired issue: althought the RD-build.pl run successfully, the reference.database.txt was empty. And the only possible message I can find was the "sort: 多字符标签“$\t”" in log file (see blow)