swiftbiosciences / primerclip

Swift Accel-Amplicon primer trimming tool for fast alignment-based primer trimming
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WARNING: 174 of 174 primer BEDPE records failed to parse (see bedparsefails.log) #12

Open iqra63 opened 6 months ago

iqra63 commented 6 months ago

Hi there, I am facing this issue with primerclip regarding parsing warning (warning snippet is attached below). primerclip is posing this warning on BED file format. I checked the primer BED file format accuracy and I guess it is perfectly fine to be executed by primerclip.

warninggg

My command is: primerclip -b masterfile/primers.bed sorted_SAM/sample_sorted.sam primerclip_result/sample_trimmed.sam

Consequently, we have the output .sam file which is not trimmed by primerclip at all. Kindly enlighten me if there is any version issue. I am trying to run primerclip on Ubuntu 22.04.3 operating system.