Metadata Attribution for scRNAseq Metacells

[fadichoucha]

Hello,

I have a question regarding the use of metadata for scRNAseq with the newly generated metacells. I recently used Metacells V0.8 to generate the metacells, and then I converted the results to v0.9. In an attempt to visualize and edit the annotation of the results, I used MCView (v0.2.9.9). While I didn't encounter any issues with cell types, I'm currently struggling to attribute metadata information, such as the Tumor sample, to the new metacells (see attached fig, at the bottom: Tumor clustering). I would appreciate any information or ideas on how to accomplish this.

Thank you for your help.

[orenbenkiki]

Do you mean how to transfer annotation metadata (such as cemm type annotation) from the old metacells to the new ones?

One way could be to:

• convey_group_to_obs to apply the old metacells data to all the cells, and then
• convey_obs_to_group to apply the per-cell data to the new metacells

This isn't "great" but it would give you a starting point. You would still need to review the per-new-metacell annotations and correct them where necessary.

[fadichoucha]

@orenbenkiki thank you for your response.

I need to transfer annotation metadata from cells meatdata to new metacells.

From your answer I think _convey_obs_togroup would help me for that.

[orenbenkiki]

Yes, that's the one.