Error: names in input file does not match with design file

[user9301]

Hello,

I am trying to analyse data using amica: DIA. I uploaded the .pg matrix and design and contrast file in the required format. However, I keep getting error the names of my samples doesn't match in input file and design file. I have tried several times by changing the names as well. Could you please help me where I am going wrong? I have attached the files from two trials (sample names are different in boh the trials). I changed the pg matrix file to .txt as I cannot upload .tsv file here. contrasts_trial1.txt design_trial1.txt contrasts_trial2.txt design_trial2.txt report.pg_matrix.tsv__trial2.txt

Thank you

[tbaccata]

Hello,

In the design_trial1.txt file there is an additional trailing whitespace in the sample E:\\projects\\p29033\\tkoiWATProteome\\20240201_016_S633866_iWAT_FL2_DIA.d. This is why you get the error of the mismatching samples in the input file.

The 2nd design design_trial2.txt would only work, if you renamed the .d column names accordingly.

Best, Sebastian

[user9301]

Hello,

Thank you for your reply. I tried changing the names in the matrix file and running it again. It still didn't work. Could you please have a look? report.pg_matrix.tsv__trial2.txt contrasts_trial2.txt design_trial2.txt

[tbaccata]

There was a trailing white space after FL2 in design_trial2.txt. If you remove it amica can process it.

Best, Sebastian

[user9301]

Thank you. It worked :)