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tbepler / topaz

Pipeline for particle picking in cryo-electron microscopy images using convolutional neural networks trained from positive and unlabeled examples. Also featuring micrograph and tomogram denoising with DNNs.
GNU General Public License v3.0
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Can denoising be applied in a particle-wise situation? #137

Closed zhaotf16 closed 2 years ago

zhaotf16 commented 2 years ago

Thanks for your contribution. The denoising algorithm has been proved in many examples and has shown good performance. I have a small question.

Say, if we have a lot of particles instead of micrographs, (like several mrcs files ). Will this still work well?

Thanks!

tbepler commented 2 years ago

Topaz denoise can be run on particle stacks and seems to work fine, but, in my experience, it generally works better to denoise the whole micrographs and then get the particle stacks from those if you want denoised particles.