Pipeline for particle picking in cryo-electron microscopy images using convolutional neural networks trained from positive and unlabeled examples. Also featuring micrograph and tomogram denoising with DNNs.
GNU General Public License v3.0
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topaz convert: when image_name is numbers like "001", one error #174
Traceback (most recent call last):
File "/home/ubuntu/.conda/envs/topaz/bin/topaz", line 33, in
sys.exit(load_entry_point('topaz-em==0.2.5', 'console_scripts', 'topaz')())
File "/home/ubuntu/.conda/envs/topaz/lib/python3.6/site-packages/topaz/main.py", line 148, in main
args.func(args)
File "/home/ubuntu/.conda/envs/topaz/lib/python3.6/site-packages/topaz/commands/convert.py", line 217, in main
file_utils.write_coordinates(output, coords, format=to_form, boxsize=boxsize, image_ext=image_ext, suffix=suffix)
File "/home/ubuntu/.conda/envs/topaz/lib/python3.6/site-packages/topaz/utils/files.py", line 198, in write_coordinates
write_via_csv(path, table)
File "/home/ubuntu/.conda/envs/topaz/lib/python3.6/site-packages/topaz/utils/files.py", line 81, in write_via_csv
filename = table['image_name'].apply(lambda x: x + '.png') # need to add .png extension
File "/home/ubuntu/.conda/envs/topaz/lib/python3.6/site-packages/pandas/core/series.py", line 4213, in apply
mapped = lib.map_infer(values, f, convert=convert_dtype)
File "pandas/_libs/lib.pyx", line 2403, in pandas._libs.lib.map_infer
File "/home/ubuntu/.conda/envs/topaz/lib/python3.6/site-packages/topaz/utils/files.py", line 81, in
filename = table['image_name'].apply(lambda x: x + '.png') # need to add .png extension
TypeError: unsupported operand type(s) for +: 'int' and 'str'
to solve this problem, modify the code on line 169 in topaz/utils/files.py:
particles.txt like this:
image_name x_coord y_coord score
002 83 100 3.9545307
002 934 594 3.4888413
002 684 667 3.4556582
when i use command :
topaz convert ./particles.txt --verbose 1 --output ./particles.csv
Traceback (most recent call last): File "/home/ubuntu/.conda/envs/topaz/bin/topaz", line 33, in
sys.exit(load_entry_point('topaz-em==0.2.5', 'console_scripts', 'topaz')())
File "/home/ubuntu/.conda/envs/topaz/lib/python3.6/site-packages/topaz/main.py", line 148, in main
args.func(args)
File "/home/ubuntu/.conda/envs/topaz/lib/python3.6/site-packages/topaz/commands/convert.py", line 217, in main
file_utils.write_coordinates(output, coords, format=to_form, boxsize=boxsize, image_ext=image_ext, suffix=suffix)
File "/home/ubuntu/.conda/envs/topaz/lib/python3.6/site-packages/topaz/utils/files.py", line 198, in write_coordinates
write_via_csv(path, table)
File "/home/ubuntu/.conda/envs/topaz/lib/python3.6/site-packages/topaz/utils/files.py", line 81, in write_via_csv
filename = table['image_name'].apply(lambda x: x + '.png') # need to add .png extension
File "/home/ubuntu/.conda/envs/topaz/lib/python3.6/site-packages/pandas/core/series.py", line 4213, in apply
mapped = lib.map_infer(values, f, convert=convert_dtype)
File "pandas/_libs/lib.pyx", line 2403, in pandas._libs.lib.map_infer
File "/home/ubuntu/.conda/envs/topaz/lib/python3.6/site-packages/topaz/utils/files.py", line 81, in
filename = table['image_name'].apply(lambda x: x + '.png') # need to add .png extension
TypeError: unsupported operand type(s) for +: 'int' and 'str'
to solve this problem, modify the code on line 169 in topaz/utils/files.py:
change
particles = pd.read_csv(path, sep='\t')
toparticles = pd.read_csv(path, sep='\t', dtype={'image_name': str})