gdadade
commented
3 years ago | Field | Value |
|---|---|
| Term | Environmental DNA |
| Definition | The physical result of DNA extraction of an environmental sample containing DNA of multiple taxa. Often completely consumed during sequencing |
| Examples | eDNA |
| Reference | Droege,G. et al. (2016), https://doi.org/10.1093/database/baw125 |
| DwC:materialSample | DNA |
| ABCD:KindOfUnit | DNA |
| GGBN:materialSampleType | DNA |
| DwC:preparations | tbd |
| ABCD:preparationType | ABCD uses GGBN term |
| GGBN:preparationType | gDNA |
| Comment | GGBN developed a preparation vocabulary including date, person, method, materials and type of preparation, hence the new more precise term "preparationType" |
| or | Field | Value |
|---|---|---|
| Term | Environmental DNA | |
| Definition | DNA from an environmental sample, e.g. soil, water, air or host organism. An often used definition is that environmental DNA is the genetic material (DNA) obtained from environmental samples without any obvious evidence of biological source material | |
| Examples | eDNA | |
| Reference | GBIF guide, https://doi.org/10.35035/doc-vf1a-nr22; Thomsen and Willerslev 2015, https://doi.org/10.1016/j.biocon.2014.11.019 |
| or | Field | Value |
|---|---|---|
| Term | Environmental DNA | |
| Definition | Refers to DNA deposited in the environment through excretion, shedding, mucous secretions, saliva etc. This can be collected in environmental samples (e.g. water, sediment) and used to identify the organisms that it originated from. eDNA in water is broken down by environmental processes over a period of days to weeks. It can travel some distance from the point at which it was released from the organism, particularly in running water. eDNA in soil can bind to organic particles and persist for a very long time (sometimes hundreds or thousands of years). eDNA is sampled in low concentrations and can be degraded (i.e. broken into short fragments), which limits the analysis options. | |
| Examples | eDNA | |
| Reference | https://www.naturemetrics.co.uk/info-hub/edna-glossary/ |
| or | Field | Value |
|---|---|---|
| Term | Environmental DNA | |
| Definition | DNA that is collected from an environment, rather than from an organism. Most commonly, this term is used to describe DNA that is no longer located in living cells (e.g. excreted DNA and DNA within cellular debris), although frequently it is also used to describe DNA extracted from biotic media (e.g. hair, blood, scat, fur). Environmental DNA is typically derived from nuclear and mitochondrial DNA and, generally, mitochondrial DNA is preferred for species detection because it is more abundant than nuclear DNA, and more sequence data are available. | |
| Examples | eDNA | |
| Reference | https://edna.epri.com/glossary.html |
| or | Field | Value |
|---|---|---|
| Term | Environmental DNA | |
| Definition | Environmental DNA refers to DNA that can be extracted from environmental samples (such as soil, water or air), without first isolating any target organisms. It is characterized by a complex mixture of genomic DNA from many different organisms and by possible degradation (i.e. DNA molecules are cut into small fragments). Total eDNA contains cellular DNA originating from living cells or organisms, and extracellular DNA (Levy‐Booth et al. 2007; Pietramellara et al. 2009) resulting from natural cell death and subsequent destruction of cell structure. | |
| Examples | eDNA | |
| Reference | Taberlet et al. 2012: Environmental DNA, https://doi.org/10.1111/j.1365-294X.2012.05542.x |