terrimporter / MetaWorks

MetaWorks is a flexible multi-marker metabarcode pipeline for processing paired-end Illumina reads from raw fastq.gz files to taxonomic assignments.
https://terrimporter.github.io/MetaWorksSite/
GNU General Public License v3.0
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vsearch versioning #6

Closed mlbowser closed 11 months ago

mlbowser commented 1 year ago

The version of vsearch included in the MetaWorks_v1.11.0 conda environment ( v2.15.2) does not implement --fastx_uniques in the dereplicate rule in the snakefile_ESV file. This was not implemented in vsearch until version 2.20.0 (see https://github.com/torognes/vsearch/issues/348).

terrimporter commented 1 year ago

I recently updated the environment file to include the correct version of VSEARCH, can you download it again, it should work fine now.

mlbowser commented 1 year ago

Thank you, @terrimporter! Thank you, also, for maintaining, updating, and making MetaWorks available.