tgen / phoenix

Jetstream compatible workflow template supporting comprehensive analysis of human sequencing data against GRCh38
MIT License
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No Duplicate stats in LIMS for BAMs with multiple Libraries #421

Open PedalheadPHX opened 3 years ago

PedalheadPHX commented 3 years ago

When we have two libraries in a bam Samtools does not provide stats on each library so we only have SM level stats but can't push to the individual LB in the LIMS. One option would be to run markdup on each library, then merge the multiple libraries so you could get stats on each LB like we will do for each PU in the planned PCR-free workflow. Alternatively, hopefully the samtools developers will address this limitation (https://github.com/samtools/samtools/issues/1143)