Closed PedalheadPHX closed 1 year ago
I'll take a look at this and see about creating a JSON from the output.
Would we want only the count of reads - e.g. we could simply run samtools view -c ubam_after_correction.u.bam
or would we want additional metrics (though since these are u.bam files we may not need or want much else at this level). We may want to run CollectDuplexSeqMetrics
as well if duplex was enabled - but that could be downstream and part of the bam_qc tasks.
yep just a simple samtools view -c input.bam
Just want to see FASTQ_Reads=100 POST_UMI_CORRECTION_Reads=80 POST_READ_COLLAPSING_Reads=64
Percent mapped currently gives some insight but not clear at what step we are losing reads
Can we collect the count of reads in the uBAM after barcode correction and the number of reads in the uBAM after simplex or duplex read collapsing @bryce-turner
@denriquez Can we capture these two into the LIMS and also the read family counts