I am not getting any alignments when using DALIGNER as a mapper. It works great when I align PacBio reads among themselves, but when I try to align them to a DAM built from the human reference sequence (hg38.fasta), I get the output below. The output of DBstats on both (dusted) databases, looks reasonable in both the number of contigs and their length distribution. I also tried mapping the reads to a smaller smaller portion of hg38 (chr1), and also tried to map chr1.dam to itself but had the same (lack of) results.
############# DALIGNER OUTPUT ##############
$ daligner -vbA -M60 $READS.dam hg38.dam
Building index for 030915_HK_run_42246_9
Kmer count = 704,510,721
Using 21.00Gb of space
Index occupies 10.41Gb
Building index for c(030915_HK_run_42246_9)
Kmer count = 704,510,721
Using 21.00Gb of space
Index occupies 10.39Gb
Building index for hg38
Comparing c(030915_HK_run_42246_9) to hg38
0 14-mers (0.000000e+00 of matrix)
0 seed hits (0.000000e+00 of matrix)
0 confirmed hits (0.000000e+00 of matrix)
Building index for hg38
Comparing 030915_HK_run_42246_9 to hg38
0 14-mers (0.000000e+00 of matrix)
0 seed hits (0.000000e+00 of matrix)
0 confirmed hits (0.000000e+00 of matrix)
I am not getting any alignments when using DALIGNER as a mapper. It works great when I align PacBio reads among themselves, but when I try to align them to a DAM built from the human reference sequence (hg38.fasta), I get the output below. The output of DBstats on both (dusted) databases, looks reasonable in both the number of contigs and their length distribution. I also tried mapping the reads to a smaller smaller portion of hg38 (chr1), and also tried to map chr1.dam to itself but had the same (lack of) results.