Open cimendes opened 4 months ago
I think we also need to consider updating String? kraken_target_organism
as it is not the organism name but rather the proportion, i.e. to String? kraken_target_organism_prop
I ran the standalone Kraken2_PE_PHB workflow here: https://app.terra.bio/#workspaces/theiagen-training-workspaces/Theiagen_Otieno_Sandbox/job_history/74a5a714-c180-4baa-b8d0-c0b266c8dccc
I used the kraken database `"gs://theiagen-large-public-files-rp/terra/databases/kraken2/k2_standard_08gb_20240112.tar.gz"
Observing some interesting results:
RSV-A reads are preferentially classified under the species Orthopneumovirus hominis
and sub-species S1 Human orthopneumovirus
whereas RSV-B reads are prefenetially classified under the species Orthopneumovirus bovis
and sub-species S1 Bovine orthopneumovirus
and S2 Respiratory syncytial virus
Some Literature from: https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-79-12-2939 Based on G-gene, the most diverse in RSV, a comparison between HRSV and BRSV revealed similarities of 38–41% at the nucleotide level and 27–32% at the amino acid level. Based on the F-gene, also diverse but more conserved than the G-gene, the similarities between HRSV and BRSV were approximately 67–71%. The other genes are likely to be even of higher similarity than the F.
Pending discussions on whether to update the docker image that includes an embedded database, or have the docker image separate from the database
:bug:
:pencil: Describe the Issue
Copied from #436
:repeat: How to Reproduce
miniwdl
orcromwell
?:fishing_pole_and_fish: Expected Behavior
:floppy_disk: Version Information
:information_source: Additional Information