Open kapsakcj opened 1 month ago
Here's an example qc_check input TSV that I used successfully with sars-cov-2 samples and the TheiaCoV_Illumina_PE workflow. The QC values are pretty lax, they could be adjusted.
The other thing that I will note that is important is that for first column taxon
, it is important to have the syntax/spelling match what is used in the theiacov organism
input parameter.
So for example, if setting up the qc_check table for West Nile Virus, the user should use WNV
in both the first column of qc_check table use "WNV"
for the organism
input parameter.
Terra test with various TheiaCoV organism input and the template qc check table here: https://app.terra.bio/#workspaces/theiagen-training-workspaces/Theiagen_Otieno_Sandbox/job_history/19ffbacf-90f8-4069-8ffc-ad744d03b94d
Some samples expected to fail read screen, others expected to run to the end.
Holding onto the issue as the docs get migrated to GitHub
:cool:
:pushpin: Explain the Request
The TheiaCoV workflow documentation page is missing a section on the optional QC_check task. Here's the v2.1.0 TheiaCoV page: https://theiagen.notion.site/TheiaCoV-Workflow-Series-47e3269a932343be982507d72fcb0fbe
It exists on the TheiaProk docs page, but not on TheiaCoV docs page.
The qccheck task is implemented in TheiaCoV ONT, ClearLabs, ILMN PE, ILMN SE, and FASTA
It would be good to have a section describing the task and additionally host a template TSV that users can use with TheiaCoV with relevant QC parameters specific to different organisms (sars-cov-2, Flu, Mpox, etc.)