I've started to use destiny to analyze sc-RNAseq data where I have WT and a treated sample, and I'm expecting that the treatment will affect the developmental trajectories.
I've used the same set of genes to perform diffusionMap on WT and the treated sample. And I see difference there.
But is there a way to project the treated sample onto diffusion components calculated based on the WT as a reference, for more of a direct comparison?
Hi!
I've started to use destiny to analyze sc-RNAseq data where I have WT and a treated sample, and I'm expecting that the treatment will affect the developmental trajectories.
I've used the same set of genes to perform diffusionMap on WT and the treated sample. And I see difference there.
But is there a way to project the treated sample onto diffusion components calculated based on the WT as a reference, for more of a direct comparison?
Thank you in advance!