In this setting, can I measure proportion of unspliced & spliced RNA, and apply it to scVelo?
To calculate the RNA velocity, we need to calculate the proportion.
And, this method is commonly used to analyse scRNA-seq dataset that have poly A tail.
But, my visium data is derived from probe-captured library.
So, I don’t think I can get the unspliced & spliced mRNA proportion.
Dear scVelo team I’m a user of scVelo.
I have a question. I have a visium sequenced data and I attached the detailed information.
https://www.10xgenomics.com/support/spatial-gene-expression-ffpe/documentation/workflows/cytassist-ffpe/steps/experimental-design-and-planning
In this setting, can I measure proportion of unspliced & spliced RNA, and apply it to scVelo?
To calculate the RNA velocity, we need to calculate the proportion. And, this method is commonly used to analyse scRNA-seq dataset that have poly A tail.
But, my visium data is derived from probe-captured library.
So, I don’t think I can get the unspliced & spliced mRNA proportion.
What do you think about that?
Many thanks.