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thermofisherlsms / iapi

Instrument Application Programming Interface
MIT License
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Question - Fusion quadrupole isolation #18

Closed chhh closed 6 years ago

chhh commented 6 years ago

If I do an MS1 scan in orbi is the quadrupole used to filter out the ions that are outside the scan mass range? Will the instrument behave differently if I do the following two scans:

["Analyzer" => "Orbitrap", "FirstMass" => 800, "LastMass" => 1000, "IsolationMode" => "None"]
["Analyzer" => "Orbitrap", "FirstMass" => 800, "LastMass" => 1000, "IsolationMode" => "Quadrupole"]
dbaileychess commented 6 years ago

When you specify 'Quadrupole' isolation, the quad will be switched to resolving mode and isolate the given mass range (FirstMass, LastMass). When the option is set to 'None', the quad is in RF-only mode and passes all masses through.