I am trying to tun the code from the Nature methods, but I get the following error:
Traceback (most recent call last):
File "de_novo_reference_creation/mergeBSv3.py", line 364, in <module>
sam_record = create_SAM_line(cluster_instance)
File "de_novo_reference_creation/mergeBSv3.py", line 225, in create_SAM_line
record.mapq = len(cluster_obj.read.seq)
File "pysam/libcalignedsegment.pyx", line 2172, in pysam.libcalignedsegment.AlignedSegment.mapq.__set__ (pysam/libcalignedsegment.c:23751)
File "pysam/libcalignedsegment.pyx", line 915, in pysam.libcalignedsegment.AlignedSegment.mapping_quality.__set__ (pysam/libcalignedsegment.c:11810)
OverflowError: value too large to convert to uint8_t
I am trying to tun the code from the Nature methods, but I get the following error:
The command, I am running is:
With
It also appears, when there is no "/1".
Any idea why this error occurs?