tilschaef / scRNA-seq

From fastq to preprocessed counttable (for in-house CELSeq2 method), with Kallisto | Bustools workflow.
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Parameter extract_meta_columns #9

Closed Rebecza closed 3 years ago

Rebecza commented 3 years ago

I use the extract_meta_columns to generate a meta data table from my cell names.

For this variable, I always add well as the last field: since in the loading of the matrix, the well-id is added (with '_') to the cell names. Is there a way to make this standard? Because, one would not expecting that to be the last field when filling in the parameters (or config file), since the folder names do not include this field as well.

Maybe the cell name extraction in general, is too specific a method to my and only some other researcher's convention of writing the plates/columns, and therefore this is not worth looking into.

However, I was thinking:

tilschaef commented 3 years ago

I see, yes we can make it standard that well and library are always appended to vector of meta_columns.

However, i think that is indeed a very specific application and we have now 3 different ways to add meta data information :).

tilschaef commented 3 years ago

a well/barcode column is now automatically added to the meta data and the user does not have to specify it explicitly.