Hello, I used sinto filterbarcodes to split CellRanger's position-sorted bam file in multiprocess mode.
Even though there is a "SO:coordinate" tag in the output bam file, I would like to know if the contents of bam are still sorted by positions/coordinates, given the parallelization of the splitting and merging.
timoast
commented
2 months ago
Hello, I used
sinto filterbarcodesto split CellRanger's position-sorted bam file in multiprocess mode.Even though there is a "SO:coordinate" tag in the output bam file, I would like to know if the contents of bam are still sorted by positions/coordinates, given the parallelization of the splitting and merging.