Closed hl-xue closed 2 years ago
Hello,
All files are processed in the same way without special consideration, except that kmtricks uses the canonical forms of k-mers.
Sorry, I just found I missed your reply 5 months ago... Thanks a lot ! Best wishes.
Hello,
I have a question about kmtricks on pair-end RNA-seq data: if the two fastq files are in "reverse-forward" mode, i.e. the first fastq contains reverse reads and the second fastq contains forward reads, does kmtricks treate them specially (for example firstly reverse-complement the first fastq before counting k-mers) ?
Thanks and best wishes !