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torognes / vsearch

Versatile open-source tool for microbiome analysis
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support for Illumina RTA3 simplified quality scores? #474

Closed frederic-mahe closed 2 years ago

frederic-mahe commented 2 years ago

(issue created to keep a record of a previous discussion)

RTA3 uses a simplified quality-scoring table with only four possible values: 2, 12, 23 and 37. This is a subset of the old range (0 to 41), so vsearch supports this simplified scoring natively, without any modification.

frederic-mahe commented 2 years ago

Tests were added to out test suite to confirm that (https://github.com/frederic-mahe/vsearch-tests/commit/30fc3fa0c4952facc6b2c2a430e2cb5ab52f60b4)