tpoorten / dotPlotly

Generate an interactive dot plot from mummer or minimap alignments
MIT License
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Issue executing pafCoordsDotPlotly.R #20

Open jsromanowski opened 1 year ago

jsromanowski commented 1 year ago

Hello all,

I have downloaded pafCoordsDotPlotly.R and have created a .paf file with my two .fa files using minimap2. I am now trying to create my dot plot, however I am having issues executing pafCoordsDotPlotly.R in my terminal.

I used the following command:

"pafCoordsDotPlotly.R -i AaegL5.chrom1.minimap2.paf -o AaegL5.chrom1.plot -s -t 95"

I receive the following error:

"pafCoordsDotPlotly.R: command not found"

note: I am operating in the directory where the pafCoordsDotPlotly.R script is saved.

Any idea how to fix this? Thanks

emistasis commented 5 months ago

I know this issue is almost a year old, but I figured I'd drop in with a possible way to resolve it in case any future users run into it.

My recommendation is to just run the script within RStudio. Make sure that you install the necessary packages beforehand, and then you should be able to run the script as follows:

install.packages(c("optparse", "ggplot2", "plotly"))

DotPlotly_lines <- paste("Rscript",
                         "pafCoordsDotPlotly.R",
                         "-i input.paf",
                         "-o output",
                         "-s",
                         "-t")
system(DotPlotly_lines)

Hope this helps!

lonelyjoeparker commented 5 months ago

It's a decent workaround. Would be helpful if the OP @jsromanowski posted OS and shell information to reproduce the error tho, plus ideally whether they've been able to run the example files and it's just their .paf which aren't working.

lonelyjoeparker commented 5 months ago

@emistasis if you're in contact with @tpoorten could you ask them to have a look at my PR https://github.com/tpoorten/dotPlotly/pull/23 for issue #18 ? Ta!