BUSCO analysis of assembly

transXpress / transXpress-nextflow

transXpress: a Nextflow pipeline for rapid de novo transcriptome assembly and annotation

GNU General Public License v3.0

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BUSCO analysis of assembly #5

Open photocyte opened 5 years ago

photocyte commented 5 years ago

BUSCO is a standard gene-content analysis program, and is quite informative. The dependencies + integration might be kind of a bummer, but there is a provided VM (https://busco.ezlab.org) which perhaps could be run through Docker.

photocyte commented 5 years ago

See:

https://gitlab.com/ezlab/busco/issues/79 -> https://gitlab.com/ezlab/busco_usecases/tree/master/phylogenomics

This Dockerfile may be more recently updated/robust: https://github.com/chrishah/BUSCO-docker

photocyte commented 5 years ago

Implemented this with 43305742bbb55845a7fe82b9ee926e7c404d88cb . Currently all hardcoded for analysis on WI hardware, but works just fine through Docker (tested on my laptop), so that seems a good solution to make it more generally accessible.