SAMSA pipeline, version 2.0. An open-source metatranscriptomics pipeline for analyzing microbiome data, built around DIAMOND and customizable reference databases.
R script to visualize differential expression between control and experimental metatranscriptomes. Takes a DESeq output file from the working directory and produces a volcano plot as a .pdf in the same directory. The R package EnhancedVolcano v1.4.0 (https://github.com/kevinblighe/EnhancedVolcano) needs to be installed beforehand on R v>3.6.1. Special characters (e.g. apostrophes) may be the source of issues with the labels on the plot. Also, need to find a way to fit the xlim in a better way.
Adding volcano plot R script created by Benoit Bechade (@benbechade). R script is tested and works with run_DESeq_stats.R output; will need to add EnhancedVolcano to list of R package dependencies.
R script to visualize differential expression between control and experimental metatranscriptomes. Takes a DESeq output file from the working directory and produces a volcano plot as a .pdf in the same directory. The R package EnhancedVolcano v1.4.0 (https://github.com/kevinblighe/EnhancedVolcano) needs to be installed beforehand on R v>3.6.1. Special characters (e.g. apostrophes) may be the source of issues with the labels on the plot. Also, need to find a way to fit the xlim in a better way.