Can you please help me with this error? If I choose the ncbi_16S_18S database, the workflow works just fine. But I need the ITS information since I sequenced this region.
Error executing process > 'prepare_databases:download_reference_ref2taxid'
Caused by:
Process prepare_databases:download_reference_ref2taxid terminated with an error exit status (4)
Command error:
--2024-06-14 09:15:02-- https://ont-exd-int-s3-euwst1-epi2me-labs.s3.amazonaws.com/wf-metagenomics/ncbi_16s_18s_28s_ITS/ncbi_16s_18s_28s_ITS.fna
Resolving ont-exd-int-s3-euwst1-epi2me-labs.s3.amazonaws.com (ont-exd-int-s3-euwst1-epi2me-labs.s3.amazonaws.com)... failed: Temporary failure in name resolution.
wget: unable to resolve host address 'ont-exd-int-s3-euwst1-epi2me-labs.s3.amazonaws.com'
cp: '.command.out' and '.command.out' are the same file
cp: '.command.err' and '.command.err' are the same file
cp: '.command.trace' and '.command.trace' are the same file
Work dir:
/home/dnasec/epi2melabs/instances/wf-metagenomics_01J0B0EKRWZ2PTPV54QKC9WC68/work/4b/619e8ee056ed47939925b0a70728e5
Tip: you can try to figure out what's wrong by changing to the process work dir and showing the script file named .command.sh
Can you please help me with this error? If I choose the ncbi_16S_18S database, the workflow works just fine. But I need the ITS information since I sequenced this region.
Error executing process > 'prepare_databases:download_reference_ref2taxid'
Caused by: Process
prepare_databases:download_reference_ref2taxid
terminated with an error exit status (4)Command executed:
wget 'https://ont-exd-int-s3-euwst1-epi2me-labs.s3.amazonaws.com/wf-metagenomics/ncbi_16s_18s_28s_ITS/ncbi_16s_18s_28s_ITS.fna' wget 'https://ont-exd-int-s3-euwst1-epi2me-labs.s3.amazonaws.com/wf-metagenomics/ncbi_16s_18s_28s_ITS/ref2taxid.ncbi_16s_18s_28s_ITS.tsv' mkdir ncbi_16s_18s_28s_ITS_db mv "ncbi_16s_18s_28s_ITS.fna" ncbi_16s_18s_28s_ITS_db/ mv "ref2taxid.ncbi_16s_18s_28s_ITS.tsv" ncbi_16s_18s_28s_ITS_db/
Command exit status: 4
Command output: (empty)
Command error: --2024-06-14 09:15:02-- https://ont-exd-int-s3-euwst1-epi2me-labs.s3.amazonaws.com/wf-metagenomics/ncbi_16s_18s_28s_ITS/ncbi_16s_18s_28s_ITS.fna Resolving ont-exd-int-s3-euwst1-epi2me-labs.s3.amazonaws.com (ont-exd-int-s3-euwst1-epi2me-labs.s3.amazonaws.com)... failed: Temporary failure in name resolution. wget: unable to resolve host address 'ont-exd-int-s3-euwst1-epi2me-labs.s3.amazonaws.com' cp: '.command.out' and '.command.out' are the same file cp: '.command.err' and '.command.err' are the same file cp: '.command.trace' and '.command.trace' are the same file
Work dir: /home/dnasec/epi2melabs/instances/wf-metagenomics_01J0B0EKRWZ2PTPV54QKC9WC68/work/4b/619e8ee056ed47939925b0a70728e5
Tip: you can try to figure out what's wrong by changing to the process work dir and showing the script file named
.command.sh