trinityrnaseq / trinityrnaseq

Trinity RNA-Seq de novo transcriptome assembly
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Trinity Phase 2 Error: --no_salmon #1331

Open FaithBoyer opened 1 year ago

FaithBoyer commented 1 year ago

Hello,

I've attached a screenshot of my Trinity script:

image

When running Trinity, I run into an issue at Phase 2: Assembling Clusters of Reads. I've attached a screenshot of this issue:

image

Could you please provide any insights on what may be the issue causing this problem? Thank you very much in advance.

brianjohnhaas commented 1 year ago

Hi,

Your wildcards in the command line are throwing off something. If you have multiple input fastq files for the left and right parameters, they'd need to be comma-delimited with no spaces. It's best to use the --samples_file parameter in that case too.

hope this helps,

~b

On Mon, Aug 14, 2023 at 3:35 PM FaithBoyer @.***> wrote:

Hello,

I've attached a screenshot of my Trinity script: [image: image] https://user-images.githubusercontent.com/71514998/260552517-381a9b74-8188-41c3-bb30-455bf76cc4a2.png

When running Trinity, I run into an issue at Phase 2: Assembling Clusters of Reads. I've attached a screenshot of this issue: [image: image] https://user-images.githubusercontent.com/71514998/260552374-f5e6614e-7f28-488a-aa24-7c20cf5d2d0f.png

Could you please provide any insights on what may be the issue causing this problem? Thank you very much in advance.

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