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tristan-mcrae-rochester / Multiphoton-Image-Analysis

Various tools for the analysis of multiphoton microscopic images at URMC's multiphoton lab.
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Illegal state: empty cluster in k-means #1

Open schienstockd opened 4 years ago

schienstockd commented 4 years ago

Hello,

I am trying your Spectral unmixing (LUMoS) in ImageJ.

I have a two-photon live-cell imaging timecourse in 3D. I have 4 fluorophores and try to unmix the sample by detecting 4 clusters.

I keep getting the following error message:

[ERROR] Module threw exception org.apache.commons.math4.exception.ConvergenceException: illegal state: empty cluster in k-means at org.apache.commons.math4.ml.clustering.KMeansPlusPlusClusterer.getPointFromLargestVarianceCluster(KMeansPlusPlusClusterer.java:440) at org.apache.commons.math4.ml.clustering.KMeansPlusPlusClusterer.cluster(KMeansPlusPlusClusterer.java:225) at com.mycompany.imagej.LUMoS_Spectral_Unmixing.run(LUMoS_Spectral_Unmixing.java:444) at org.scijava.command.CommandModule.run(CommandModule.java:199) at org.scijava.module.ModuleRunner.run(ModuleRunner.java:168) at org.scijava.module.ModuleRunner.call(ModuleRunner.java:127) at org.scijava.module.ModuleRunner.call(ModuleRunner.java:66) at org.scijava.thread.DefaultThreadService$3.call(DefaultThreadService.java:238) at java.util.concurrent.FutureTask.run(FutureTask.java:266) at java.util.concurrent.ThreadPoolExecutor.runWorker(ThreadPoolExecutor.java:1149) at java.util.concurrent.ThreadPoolExecutor$Worker.run(ThreadPoolExecutor.java:624) at java.lang.Thread.run(Thread.java:748)

csbbbv commented 1 year ago

Did you solve this problem? I got this error too

schienstockd commented 1 year ago

No ... Gave up on it. But it would be useful to have to clean up images.

khoroshyy commented 12 months ago

Replicate #1 [ERROR] Command errored: LUMoS Spectral Unmixing org.apache.commons.math4.exception.ConvergenceException: illegal state: empty cluster in k-means at org.apache.commons.math4.ml.clustering.KMeansPlusPlusClusterer.getPointFromLargestVarianceCluster(KMeansPlusPlusClusterer.java:440) at org.apache.commons.math4.ml.clustering.KMeansPlusPlusClusterer.cluster(KMeansPlusPlusClusterer.java:225) at com.mycompany.imagej.K_Means.run(K_Means.java:444) at org.scijava.command.CommandModule.run(CommandModule.java:196) at org.scijava.module.ModuleRunner.run(ModuleRunner.java:165) at org.scijava.module.ModuleRunner.call(ModuleRunner.java:125) at org.scijava.module.ModuleRunner.call(ModuleRunner.java:64) at org.scijava.thread.DefaultThreadService.lambda$wrap$2(DefaultThreadService.java:247) at java.util.concurrent.FutureTask.run(FutureTask.java:266) at java.util.concurrent.ThreadPoolExecutor.runWorker(ThreadPoolExecutor.java:1149) at java.util.concurrent.ThreadPoolExecutor$Worker.run(ThreadPoolExecutor.java:624) at java.lang.Thread.run(Thread.java:750) I get this one