Closed EricFournier3 closed 4 years ago
@EricFournier3 it probably will not work because it relies in in silico PCR so if you don't have the primers in the template you sequenced it won't be able to find the amplicons? The primers are essential to distinguising the mompS
paralog.
However, you could make an mlst
database from the SBT files and use mlst
because that uses the sequence alone.
Hi Torsen, i am wondering if the script works fine with concatenation of the 7 sequences in one fasta file instead of a whole genome sequence. We have only data from first generation sequencing. Actually, i tried it and it doesn't works.
Thanks Eric