I am having troubbles to MLST a Campylobacter isolate. The problem is that your tool cannot identify ST-10652 and I found this is occuring due to a mismatching for the allele glyA between your tool and PubMLST. Your tool finds glyA 83 whereas PubMLST identifies glyA 849 and so ST is 10652 when using directly PubMLST, but your tool can't find this ST.
I BLAST the sequence of glyA 849 https://pubmlst.org/bigsdb?db=pubmlst_campylobacter_seqdef&page=alleleInfo&locus=glyA&allele_id=849 against my genome and the sequence is identical to this allele, but not to glyA 83.
I am having troubbles to MLST a Campylobacter isolate. The problem is that your tool cannot identify ST-10652 and I found this is occuring due to a mismatching for the allele glyA between your tool and PubMLST. Your tool finds glyA 83 whereas PubMLST identifies glyA 849 and so ST is 10652 when using directly PubMLST, but your tool can't find this ST. I BLAST the sequence of glyA 849 https://pubmlst.org/bigsdb?db=pubmlst_campylobacter_seqdef&page=alleleInfo&locus=glyA&allele_id=849 against my genome and the sequence is identical to this allele, but not to glyA 83.
What is going wrong?