Closed foamy1881 closed 4 years ago
why all the core alignment SNP are the same for all isolates
what do you mean?
SNP and distance matrix output most of them are 0 and 1
assuming you are using most of the reference genome in your core, this is a normal result if you have an outbreak or strongly related isolates.
quick question when nullarbor2 generate the distance matrix which file does it use from snippy? core.aln or core.full.aln?
@foamy1881 is uses core.aln
distances.tab : core.aln
snp-dists -b $< > $@
Hi finished running nullarbor2 and when I looked at the core SNP and distance matrix output most of them are 0 and 1. I read numerous post saying please removed unaligned >200,000 bp and snippy must run on isolates of more than 10x coverage. I check those and it seem fine and I manage to run snippy by itself but it still produces the same outcome. Can someone point my mistake?
I have add ".txt" at the end of the core.aln file so i can upload them. i'm puzzle why all the core alignment SNP are the same for all isolates? Maybe some stupid mistake by me? can someone help?
core.aln.txt